code used for analysis of 2p imaging data Search Results


thioflavin t puncta  (Revvity)
99
Revvity thioflavin t puncta
a Misfolded protein load in gentamicin (GENTA), tunicamycin (TUNICA), and/or GGA-exposed cells assessed by Thioflavin T staining. Red = Thioflavin T; blue = Hoechst stained nuclei; bars = 50 µm. b Thioflavin T <t>puncta</t> quantified in gentamicin or tunicamycin vs. GGA-exposed renal cells. c Gentamicin increased oxidative stress evidenced by increased steady-state 4HNE content. d GGA significantly reduced 4HNE content in gentamicin-exposed cells. e Content of ROS in renal cells after variable duration exposure to gentamicin assessed by fluorescent whole-cell oxidative stress assay. f GGA significantly reduced whole-cell ROS content in gentamicin-exposed cells; bars = 50 µm; NS = nonsignificant; * P < 0.05.
Thioflavin T Puncta, supplied by Revvity, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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andor zyla 4 2 p scmos cameras  (Oxford Instruments)
99
Oxford Instruments andor zyla 4 2 p scmos cameras
a Misfolded protein load in gentamicin (GENTA), tunicamycin (TUNICA), and/or GGA-exposed cells assessed by Thioflavin T staining. Red = Thioflavin T; blue = Hoechst stained nuclei; bars = 50 µm. b Thioflavin T <t>puncta</t> quantified in gentamicin or tunicamycin vs. GGA-exposed renal cells. c Gentamicin increased oxidative stress evidenced by increased steady-state 4HNE content. d GGA significantly reduced 4HNE content in gentamicin-exposed cells. e Content of ROS in renal cells after variable duration exposure to gentamicin assessed by fluorescent whole-cell oxidative stress assay. f GGA significantly reduced whole-cell ROS content in gentamicin-exposed cells; bars = 50 µm; NS = nonsignificant; * P < 0.05.
Andor Zyla 4 2 P Scmos Cameras, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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inverted confocal laser microscope nikon eclipse ti-e  (Nikon)
90
Nikon inverted confocal laser microscope nikon eclipse ti-e
a Misfolded protein load in gentamicin (GENTA), tunicamycin (TUNICA), and/or GGA-exposed cells assessed by Thioflavin T staining. Red = Thioflavin T; blue = Hoechst stained nuclei; bars = 50 µm. b Thioflavin T <t>puncta</t> quantified in gentamicin or tunicamycin vs. GGA-exposed renal cells. c Gentamicin increased oxidative stress evidenced by increased steady-state 4HNE content. d GGA significantly reduced 4HNE content in gentamicin-exposed cells. e Content of ROS in renal cells after variable duration exposure to gentamicin assessed by fluorescent whole-cell oxidative stress assay. f GGA significantly reduced whole-cell ROS content in gentamicin-exposed cells; bars = 50 µm; NS = nonsignificant; * P < 0.05.
Inverted Confocal Laser Microscope Nikon Eclipse Ti E, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/inverted confocal laser microscope nikon eclipse ti-e/product/Nikon
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scmos camera  (Nikon)
99
Nikon scmos camera
a Misfolded protein load in gentamicin (GENTA), tunicamycin (TUNICA), and/or GGA-exposed cells assessed by Thioflavin T staining. Red = Thioflavin T; blue = Hoechst stained nuclei; bars = 50 µm. b Thioflavin T <t>puncta</t> quantified in gentamicin or tunicamycin vs. GGA-exposed renal cells. c Gentamicin increased oxidative stress evidenced by increased steady-state 4HNE content. d GGA significantly reduced 4HNE content in gentamicin-exposed cells. e Content of ROS in renal cells after variable duration exposure to gentamicin assessed by fluorescent whole-cell oxidative stress assay. f GGA significantly reduced whole-cell ROS content in gentamicin-exposed cells; bars = 50 µm; NS = nonsignificant; * P < 0.05.
Scmos Camera, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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scmos camera - by Bioz Stars, 2026-04
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confocal microscope 63x objective zeiss lsm510 meta 2p  (Carl Zeiss)
90
Carl Zeiss confocal microscope 63x objective zeiss lsm510 meta 2p
a Misfolded protein load in gentamicin (GENTA), tunicamycin (TUNICA), and/or GGA-exposed cells assessed by Thioflavin T staining. Red = Thioflavin T; blue = Hoechst stained nuclei; bars = 50 µm. b Thioflavin T <t>puncta</t> quantified in gentamicin or tunicamycin vs. GGA-exposed renal cells. c Gentamicin increased oxidative stress evidenced by increased steady-state 4HNE content. d GGA significantly reduced 4HNE content in gentamicin-exposed cells. e Content of ROS in renal cells after variable duration exposure to gentamicin assessed by fluorescent whole-cell oxidative stress assay. f GGA significantly reduced whole-cell ROS content in gentamicin-exposed cells; bars = 50 µm; NS = nonsignificant; * P < 0.05.
Confocal Microscope 63x Objective Zeiss Lsm510 Meta 2p, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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confocal microscope 63x objective zeiss lsm510 meta 2p - by Bioz Stars, 2026-04
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2p-laser  (3i - Intelligent Imaging)
90
3i - Intelligent Imaging 2p-laser
a Misfolded protein load in gentamicin (GENTA), tunicamycin (TUNICA), and/or GGA-exposed cells assessed by Thioflavin T staining. Red = Thioflavin T; blue = Hoechst stained nuclei; bars = 50 µm. b Thioflavin T <t>puncta</t> quantified in gentamicin or tunicamycin vs. GGA-exposed renal cells. c Gentamicin increased oxidative stress evidenced by increased steady-state 4HNE content. d GGA significantly reduced 4HNE content in gentamicin-exposed cells. e Content of ROS in renal cells after variable duration exposure to gentamicin assessed by fluorescent whole-cell oxidative stress assay. f GGA significantly reduced whole-cell ROS content in gentamicin-exposed cells; bars = 50 µm; NS = nonsignificant; * P < 0.05.
2p Laser, supplied by 3i - Intelligent Imaging, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2p-laser - by Bioz Stars, 2026-04
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cytokeratin 3  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology cytokeratin 3
a Misfolded protein load in gentamicin (GENTA), tunicamycin (TUNICA), and/or GGA-exposed cells assessed by Thioflavin T staining. Red = Thioflavin T; blue = Hoechst stained nuclei; bars = 50 µm. b Thioflavin T <t>puncta</t> quantified in gentamicin or tunicamycin vs. GGA-exposed renal cells. c Gentamicin increased oxidative stress evidenced by increased steady-state 4HNE content. d GGA significantly reduced 4HNE content in gentamicin-exposed cells. e Content of ROS in renal cells after variable duration exposure to gentamicin assessed by fluorescent whole-cell oxidative stress assay. f GGA significantly reduced whole-cell ROS content in gentamicin-exposed cells; bars = 50 µm; NS = nonsignificant; * P < 0.05.
Cytokeratin 3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cytokeratin 3 - by Bioz Stars, 2026-04
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16× nikon water immersion objective  (Nikon)
90
Nikon 16× nikon water immersion objective
a Misfolded protein load in gentamicin (GENTA), tunicamycin (TUNICA), and/or GGA-exposed cells assessed by Thioflavin T staining. Red = Thioflavin T; blue = Hoechst stained nuclei; bars = 50 µm. b Thioflavin T <t>puncta</t> quantified in gentamicin or tunicamycin vs. GGA-exposed renal cells. c Gentamicin increased oxidative stress evidenced by increased steady-state 4HNE content. d GGA significantly reduced 4HNE content in gentamicin-exposed cells. e Content of ROS in renal cells after variable duration exposure to gentamicin assessed by fluorescent whole-cell oxidative stress assay. f GGA significantly reduced whole-cell ROS content in gentamicin-exposed cells; bars = 50 µm; NS = nonsignificant; * P < 0.05.
16× Nikon Water Immersion Objective, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2p imaging data  (Addgene inc)
93
Addgene inc 2p imaging data
Figure 5. Two-photon calcium imaging during iMRSIV spatial behaviors (A) <t>iMRSIV+2P.</t> Example two-photon imaging field of CA1 neurons labeled <t>with</t> <t>jGCaMP8m</t> and regions of interest (ROIs). Imaging during familiar linear track navigation using iMRSIV.
2p Imaging Data, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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evos fl auto digital microscope  (Thermo Fisher)
90
Thermo Fisher evos fl auto digital microscope
Figure 5. Two-photon calcium imaging during iMRSIV spatial behaviors (A) <t>iMRSIV+2P.</t> Example two-photon imaging field of CA1 neurons labeled <t>with</t> <t>jGCaMP8m</t> and regions of interest (ROIs). Imaging during familiar linear track navigation using iMRSIV.
Evos Fl Auto Digital Microscope, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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evos fl auto digital microscope - by Bioz Stars, 2026-04
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2p images  (MathWorks Inc)
90
MathWorks Inc 2p images
Figure 5. Two-photon calcium imaging during iMRSIV spatial behaviors (A) <t>iMRSIV+2P.</t> Example two-photon imaging field of CA1 neurons labeled <t>with</t> <t>jGCaMP8m</t> and regions of interest (ROIs). Imaging during familiar linear track navigation using iMRSIV.
2p Images, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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two-photon laser scanning microscope  (Nikon)
90
Nikon two-photon laser scanning microscope
Figure 5. Two-photon calcium imaging during iMRSIV spatial behaviors (A) <t>iMRSIV+2P.</t> Example two-photon imaging field of CA1 neurons labeled <t>with</t> <t>jGCaMP8m</t> and regions of interest (ROIs). Imaging during familiar linear track navigation using iMRSIV.
Two Photon Laser Scanning Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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two-photon laser scanning microscope - by Bioz Stars, 2026-04
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Image Search Results


a Misfolded protein load in gentamicin (GENTA), tunicamycin (TUNICA), and/or GGA-exposed cells assessed by Thioflavin T staining. Red = Thioflavin T; blue = Hoechst stained nuclei; bars = 50 µm. b Thioflavin T puncta quantified in gentamicin or tunicamycin vs. GGA-exposed renal cells. c Gentamicin increased oxidative stress evidenced by increased steady-state 4HNE content. d GGA significantly reduced 4HNE content in gentamicin-exposed cells. e Content of ROS in renal cells after variable duration exposure to gentamicin assessed by fluorescent whole-cell oxidative stress assay. f GGA significantly reduced whole-cell ROS content in gentamicin-exposed cells; bars = 50 µm; NS = nonsignificant; * P < 0.05.

Journal: Cell Death & Disease

Article Title: Cross organelle stress response disruption promotes gentamicin-induced proteotoxicity

doi: 10.1038/s41419-020-2382-7

Figure Lengend Snippet: a Misfolded protein load in gentamicin (GENTA), tunicamycin (TUNICA), and/or GGA-exposed cells assessed by Thioflavin T staining. Red = Thioflavin T; blue = Hoechst stained nuclei; bars = 50 µm. b Thioflavin T puncta quantified in gentamicin or tunicamycin vs. GGA-exposed renal cells. c Gentamicin increased oxidative stress evidenced by increased steady-state 4HNE content. d GGA significantly reduced 4HNE content in gentamicin-exposed cells. e Content of ROS in renal cells after variable duration exposure to gentamicin assessed by fluorescent whole-cell oxidative stress assay. f GGA significantly reduced whole-cell ROS content in gentamicin-exposed cells; bars = 50 µm; NS = nonsignificant; * P < 0.05.

Article Snippet: Thioflavin T puncta was measured by automated image cytometry via the Celigo Image Cytometer (Nexcelom; Lawrence, MA).

Techniques: Staining

Figure 5. Two-photon calcium imaging during iMRSIV spatial behaviors (A) iMRSIV+2P. Example two-photon imaging field of CA1 neurons labeled with jGCaMP8m and regions of interest (ROIs). Imaging during familiar linear track navigation using iMRSIV.

Journal: Neuron

Article Title: Full field-of-view virtual reality goggles for mice.

doi: 10.1016/j.neuron.2023.11.019

Figure Lengend Snippet: Figure 5. Two-photon calcium imaging during iMRSIV spatial behaviors (A) iMRSIV+2P. Example two-photon imaging field of CA1 neurons labeled with jGCaMP8m and regions of interest (ROIs). Imaging during familiar linear track navigation using iMRSIV.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains AAV9-syn-jGCaMP8m-WPRE Addgene RRID: Addgene_162375 Deposited data Processed behavior and 2p imaging data This paper Zenodo: https://doi.org/10.5281/zenodo.8393062 Experimental models: Organisms/strains C57BL/6J The Jackson Laboratory RRID: IMSR_JAX:000664 BALB/C Charles River RRID: IMSR_CRL:028 Software and algorithms Unity 2021.2.9f1 Unity Technologies https://unity.com MATLAB R2022b MathWorks https://www.mathworks.com OpticStudio 23.1.1 Zemax, LLC https://www.zemax.com Blender 3.5 The Blender Foundation https://www.blender.org Custom Unity scripts and VR environments This paper github.com/DombeckLab/IMRSIV and Zenodo: https://doi.org/10.5281/zenodo.10127394 Other Lens design in Zemax This paper github.com/DombeckLab/IMRSIV STL files for custom-printed parts This paper github.com/DombeckLab/IMRSIV

Techniques: Imaging, Labeling